Nitrative Stress-Related Autophagic Insufficiency Participates in Hyperhomocysteinemia-Induced Renal Aging

Figure 3

HHcy decreased the expression of TFEB in renal tissue. (a–f) The protein expression of TFEB in kidney extracts of HHcy rats and MPC-5 cells measured by Western blot. (a) Representative Western blot showing the protein expression of TFEB in HHcy. Graphs representing (b) TFEB by quantitative analysis (, HHcy vs. Control, -5). (c) Representative Western blot showing the protein expression of TFEB in MPC-5 treated with rapamycin. Graphs representing (d) quantitative analysis (, Hcy vs. Control, rapamycin vs. Hcy, -4). (e) Representative Western blot showing the protein expression of TFEB in MPC-5 infected with Atg5 adenovirus. Graphs representing (f) (, Hcy vs. Control, Atg5 adenovirus vs. Hcy, -4). (g) Graphs representing quantitative analysis showing the mRNA level of TFEB in MPC-5 treated with Hcy for 6, 12, and 24 h (, , and , Hcy vs. Control, n = 3-4). Control: control group; Hcy: homocysteinemia group; Ad-Atg5: Atg5 adenovirus. Data were expressed as .

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