Nitrative Stress-Related Autophagic Insufficiency Participates in Hyperhomocysteinemia-Induced Renal Aging

Figure 4

Nitrative stress participated in Hcy-induced TFEB downregulation. (a–c) Representative Western blot and immunohistochemistry detecting 3-NT contents in renal tissue of HHcy rats, respectively. (, HHcy vs. Control, -5). (d) Representative immunoprecipitation showing the contents of nitrated TFEB. Graphs representing (e) quantitative analysis (ns, , Hcy vs. Control, FeTMPyP vs. Hcy, ). (f) Representative Western blot showing the protein expression of TFEB and 3-NT in MPC-5 treated with FeTMPyP. Graphs representing (g) quantitative analysis (, Hcy vs. Control, FeTMPyP vs. Hcy, -4). (h–j) Graphs representing quantitative analysis showing the mRNA level of TFEB in MPC-5 treated with FeTMPyP for 6, 12, and 24 h (, , and , Hcy vs. Control, FeTMPyP vs. Hcy, -4). Control: control group; Hcy: homocysteinemia group. Data were expressed as .

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