Regulatory Effects of Nur77 on Airway Remodeling and ASMC Proliferation in House Dust Mite-Induced Asthma

Figure 1

HDM exposure increased lung Nur77 expression in chronic asthma model. (a) The asthmatic mouse model was induced with 50 μL of 100 μg HDM extract to the trachea 3 times per week for consecutive 4 weeks; mice were sacrificed 18-20 hrs after the last HDM exposure for further analysis. (b, d) BALF were collected for detecting the counts of total inflammatory cell, eosinophils, neutrophil, and macrophage (b) as well as Ig-E (d) production. (c) Lung sections were stained with H&E to evaluate leukocyte infiltration (first column), PAS to analyze goblet cell hyperplasia (second column), Masson’s trichrome to assess the collagen deposition and fibrosis (third column), and immunohistochemical staining for Nur77 to determine its distribution and expression (fourth column); scale bar for all images: 50 μm. (e) Quantitative analysis of mucus production in lung sections. (f, g) WB assay of Nur77 level in lung tissue affected by HDM challenge and shRNA interfere. , , and .

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