Regulatory Effects of Nur77 on Airway Remodeling and ASMC Proliferation in House Dust Mite-Induced Asthma

<div>Nur77 modulated proliferation of the primary human ASMC under HDM exposure. (a) One representative microscopic field of primary human ASMC was shown; scale bar: 200 <i>μ</i>m. (b, d) Transfection of ASMC with Nur77 shRNA downregulated its mRNA (b) and protein (d) expression at 12 h compared with control shRNA (NEGi) treatment. (c) WB assay of Nur77 in ASMC with or without HDM (80 <i>μ</i>g/mL) challenge. (e) Effect of different concentrations of HDM stimulation on ASMC proliferation via the CCK-8 assay. (f–h) Effect of Nur77i on HDM-stimulated ASMC by the methods of CCK-8 and EdU assays. Scale bar: 200 <i>μ</i>m. 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Regulatory Effects of Nur77 on Airway Remodeling and ASMC Proliferation in House Dust Mite-Induced Asthma 