Safflor Yellow B Attenuates Ischemic Brain Injury via Downregulation of Long Noncoding AK046177 and Inhibition of MicroRNA-134 Expression in Rats

Figure 5

Effect of safflor yellow B on NADPH oxidase activity and Nox4 expression. Rats and primary fetal cortical cells were used to establish the I/R model and the OGD/R model, respectively. After I/R for 24 h, the cerebral cortex and cells from different groups were collected. NADPH oxidase activity and Nox4 expression was observed using Western blot analysis. (a1) and (a2) represent the NADPH oxidase activity of the cerebral cortex and the primary fetal cortical cells, respectively. (b1) Representative western blots of the cerebral cortex are shown (1, 2, 3, 4, 5, and 6 represent sham, ischemia/reperfusion (I/R), AK046177 siRNA, AK046177 siRNA+miR-134 agomir, SYB, and SYB+miR-134 agomir, respectively). (b2) represents Nox4 expression in the cerebral cortex. (c1) Representative western blots of primary fetal cortical cells are shown (1, 2, 3, 4, 5, and 6 represent control, OGD/R, AK046177 siRNA, AK046177 siRNA+miR-134 agomir, SYB, and SYB+miR-134 agomir, respectively). (c2) represent Nox4 expression in cells. Data are presented as ( in brain tissues, or in cells). One-way ANOVA test was used to determine statistical significance. vs. the sham group or the control group, ^##vs. the I/R group or the OGD/R group, ^Δ or ^ΔΔvs. the AK046177 siRNA group, and ⁺ or ⁺⁺vs. the SYB group.

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