Overexpression of miR-506-3p Aggravates DBP-Induced Testicular Oxidative Stress in Rats by Downregulating ANXA5 via Nrf2/HO-1 Signaling Pathway

Figure 5

Recombinant rat ANXA5 reversed the testicular oxidative stress promoting injury of miR-506-3p in DBP-treated rats: (a) H&E staining of testis tissues in DBP-treated rats following Agomir-506-3p and cotransfection of recombinant rat ANXA5 and Agomir-506-3p; (b) DHE staining of testis tissues in DBP-treated rats following Agomir-506-3p and cotransfection of recombinant rat ANXA5 and Agomir-506-3p, ROS exhibit red fluorescence under fluorescent microscope; (c) immunohistochemical staining showed ANXA5 expression level of testis tissues in DBP-treated rats following Agomir-506-3p and cotransfection of recombinant rat ANXA5 and Agomir-506-3p; (d) Western blotting showed the expression levels of Nrf2, ANXA5, HO-1, NQO1, and GST proteins of testis tissues in DBP-treated rats following Agomir-506-3p and cotransfection of recombinant rat ANXA5 and Agomir-506-3p. The data are showed as . Significantly different from the DBP + Agomir-506-3p group. , .

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