Overexpression of miR-506-3p Aggravates DBP-Induced Testicular Oxidative Stress in Rats by Downregulating ANXA5 via Nrf2/HO-1 Signaling Pathway

Figure 6

miR-506-3p aggravated the oxidative stress of DBP-treated TM3 cells by downregulating ANXA5 in vitro: (a) cell viability after 24 h of 10 mg/L DBP administration after cotransfection of recombinant rat ANXA5 and miR-506-3p mimic in TM3 and TM4 cells; (b) qRT-PCR showed the expression level of miR-506-3p of testis tissues after 24 h of 10 mg/L DBP administration after cotransfection of recombinant rat ANXA5 and miR-506-3p mimic in TM3 and TM4 cells; (c) DHE staining of testis tissues after 24 h of 10 mg/L DBP administration after cotransfection of recombinant rat ANXA5 and miR-506-3p mimic in TM3 and TM4 cells; (d) Western blotting showed the expression levels of Nrf2, ANXA5, HO-1, NQO1, and GST proteins of testis tissues after 24 h of 10 mg/L DBP administration after cotransfection of recombinant rat ANXA5 and miR-506-3p mimic in TM3 and TM4 cells. The data are showed as . Significantly different from NC mimic. ^#Significantly different from miR-506-3p mimic. ^#, .

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