Baicalin Ameliorates Cognitive Impairment and Protects Microglia from LPS-Induced Neuroinflammation via the SIRT1/HMGB1 Pathway

Figure 2

BAI attenuated LPS-induced activated glial cells and the inflammatory response in the mouse hippocampus. (a) Representative photomicrographs of the immunohistochemical analysis of microglia (Iba-1-positive cells) and astrocytes (GFAP-positive cells) in the hippocampal CA1 and DG subregions of mice 24 h after LPS administration and (b) their statistical analysis (scale bars: 100 μM; ). (c) Immunoblotting was used to determine the Iba-1 and GFAP protein levels in hippocampal tissue lysates. (d) IL-1β and TNF-α expression in the hippocampus was determined by immunoblotting 24 h after LPS and BAI administration. (e) Quantification of the data shown in c and d. Data are presented as the ( per group). , , control vs. LPS; ^#, ^##, ^###, BAI+LPS vs. LPS.

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