Sulforaphene Ameliorates Neuroinflammation and Hyperphosphorylated Tau Protein via Regulating the PI3K/Akt/GSK-3β Pathway in Experimental Models of Alzheimer’s Disease

Figure 12

Effects of SF on the LPS-induced activation of GSK-3β and PI3K p110α by LPS in BV-2 cells. BV-2 cells were pretreated with SF (1 and 2 μM) for 1 h followed by treatment with LPS (1 μg/mL) for another 23 h. LY294002 (5 μM) was pretreated for 1 h followed by treatment with SF (2 μM) for another 23 h. (a) Representative immunoblot bands and quantitative analysis of p-GSK-3β (S9), GSK-3β, and β-actin in BV-2 cells. (b) Representative immunoblot bands and quantitative analysis of PI3K p110α and β-actin in BV-2 cells. (c) Representative immunoblot bands and quantitative analysis of p-GSK-3β (S9), GSK-3β, and β-actin in LY294002-treated BV-2 cells. The results were expressed as the (). and as compared with the vehicle control group; ^# and ^## as compared with the LPS-treated control group; ^&& as compared with the SF treatment group; ns as compared with the LY294002 group.

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