The HIPPO Transducer YAP and Its Targets CTGF and Cyr61 Drive a Paracrine Signalling in Cold Atmospheric Plasma-Mediated Wound Healing

Figure 4

Effect of CAP on the HIPPO signalling pathway: the relative phosphorylation level of YAP is shown in GM Fbs (a) and HaCaT cells (b). CTGF and Cyr61 protein expression was enhanced after 10 and 30 s of CAP treatment in GM Fbs (c, d). CTGF expression slightly increased after 10 and 30 s of CAP treatments in HaCaT cells after approximately 3 hours (e). Representative blots are shown. The -axis represents CAP treatment time and incubation time after plasma treatment. Data are represented as of at least three independent experiments. Statistical analysis was performed using unpaired -test with Welch’s correction for multiple comparisons, along with normalization to the untreated control.

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