GPA Peptide-Induced Nur77 Localization at Mitochondria Inhibits Inflammation and Oxidative Stress through Activating Autophagy in the Intestine

Figure 3

GPA inhibits inflammation and oxidative stress in IECs. MODE-K cells were treated with different GPA concentrations for 6 h, and the cytotoxicity was detected by LDH assay (a). MODE-K cells pretreated with different concentrations of GPA for 6 h were exposed to LPS (1 μg/ml) for 3 h, and the cytotoxicity was detected by LDH assay (b). The level of IL-6 was detected by western blot (c, d). MCP-1 and TNF-α levels were detected by ELISA (e). The intracellular ROS levels were measured by DCF fluorescence microscopy (f). And the content of MDA was determined (g). The results are expressed as the , three independent experiments. , , and vs. the control group; ^#, ^##, and ^### vs. the LPS-treated group.

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