GPA Peptide-Induced Nur77 Localization at Mitochondria Inhibits Inflammation and Oxidative Stress through Activating Autophagy in the Intestine

Figure 7

Nur77 mediates the anti-inflammatory and antioxidant effects of GPA in IECs. MODE-K cells were transfected with control or Nur77siRNA and further stimulated with GPA (1 mM) for 6 h, followed by LPS (1 μg/ml) for 3 h; levels of Nur77, IL-6, and MCP-1 were analyzed by qPCR (a, b, c). The intracellular ROS levels were measured by DCF fluorescence microscopy (d). The results are expressed as the , three independent experiments. , , and vs. the control group; ^#, ^##, and ^### vs. the LPS-treated group.

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