Lactate Induces Cisplatin Resistance in S. cerevisiae through a Rad4p-Dependent Process

Figure 5

Effect of lactate on H2A phosphorylation during cisplatin treatment. Wild-type BY4741 cells were grown to early exponential phase in SC-glucose medium (pH 5.5). Cells were washed and exposed to 600 μg/mL cisplatin in SC medium containing glucose or lactate for up to 180 minutes. H2A phosphorylation levels were assessed by Western blot. Pgk1p levels were used as a loading control. A representative image from 2 independent experiments is shown.