Lactate Induces Cisplatin Resistance in S. cerevisiae through a Rad4p-Dependent Process

Figure 6

Effect of RAD4 deletion on lactate-mediated resistance to cisplatin. Wild-type BY4741 and rad4Δ cells were grown to early exponential phase in SC-glucose medium (pH 5.5). Cells were washed and exposed to cisplatin in SC medium containing glucose or lactate for up to 180 minutes. (a) Cell viability was assessed by semiquantitative spot assay, (b) colony forming units (c.f.u.) of cells exposed to 450 μg/mL cisplatin for 120 minutes for rad4Δ and 180 minutes for wild-type BY4741, and (c) c.f.u. of cells exposed to 600 μg/mL cisplatin for 120 minutes for rad4Δ and 180 minutes for wild-type BY4741. In (b) and (c), cell viability was determined by standard dilution plate counts and expressed as a percentage of c.f.u. on YPD plates in relation to time 0. Statistical analysis by Prism 5 (two-way ANOVA and Sidak’s multiple comparisons test); . Values represent of four independent experiments.

(a)

(b)

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