Single Point Mutation from E22-to-K in Aβ Initiates Early-Onset Alzheimer’s Disease by Binding with Catalase

Figure 3

Identification of the complex structure of E22K/catalase and Aβ/catalase in vitro. (a–e) The binding strength between Aβ and catalase (0.05 mg/mL) and between E22K and catalase (0.05 mg/mL) as assessed by Coomassie Blue or by western blot (WT) analysis of catalase antibody and A11, respectively. The quantified WT band intensity of CAT after Aβ or E22K incubated with CAT (e). (f–i) Aβ (black) or E22K (black) induced the complex formation of Aβ/catalase or E22K/catalase (catalase, white balls) at 0 and 24 h (TEM). Bar: 0.2 μm. (j) Colocalization of E22K (green) and catalase (red) after E22K or Aβ incubation for 6 hours in cultured human SY5Y cells. DAPI: nuclear stain, blue. (k) Colocalization of E22K (green) and catalase (red) after E22K or Aβ injection 30 days prior. All experiments were repeated in triplicate.

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