Single Point Mutation from E22-to-K in Aβ Initiates Early-Onset Alzheimer’s Disease by Binding with Catalase

Figure 5

L-cysteine reduced the aggregation of E22K and Aβ in vitro. (a, b) The theoretical simulated 3D crystal structure of L-cys (red) binding with the F19 (purple) and D23 (yellow, full line) residues of Aβ by H-bonding (yellow dotted line). (c, d) The simulated 3D crystal structure of L-cys (red) binding with the K16 (blue) residue of E22K by H-bonding (yellow dotted line). (e–h) The effects of L-cys on β-sheet and fibril formation at 0 and 24 h as examined by Th T dye and TEM. Bar: 0.2 μm. Experiments were repeated in triplicate. . Data are the .

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