Single Point Mutation from E22-to-K in Aβ Initiates Early-Onset Alzheimer’s Disease by Binding with Catalase

Figure 6

E22K injection induced more plaque deposition and neuronal loss compared with Aβ injection. (a–d) The effects of saline (Con), Aβ, and Aβ plus L-cys on the formation of senile plaques (SPs) in the hippocampi and cortices of rats quantified by immunochemistry using antibody-4G8. (e) ECS blockage by SPs, which were doubly stained with Th S dye for SPs (green) and DAPI dye for the nuclei of neurons (blue). ECS: extracellular space. (f–i) The effects of saline (Con), E22K, and E22K plus L-cys on SP formation. Scale bar, 500 μm. sections from 3–4 rats each. (j) ECS blockage by SPs, which were doubly stained with Th S dye for SPs (green) and DAPI dye for the nuclei of neurons (blue). (k–o) The effects of saline (Con), Aβ, Aβ plus L-cys, E22K, and E22K plus L-cys on the numbers of hippocampal neurons examined by H&E staining. Scale bar, 200 μm. sections from 3–4 rats each. (p) The statistical data of the relative numbers of hippocampal neurons in the above groups. . Data are .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)