Research Article

Protective Effect of Astragaloside IV on High Glucose-Induced Endothelial Dysfunction via Inhibition of P2X7R Dependent P38 MAPK Signaling Pathway

Figure 1

Effect of As-IV on endothelium-dependent relaxation in STZ-induced diabetic rats. (a) Representative typical traces of ACh-stimulated endothelium-dependent relaxation in PE-precontracted aorta. (b, c) Endothelium-dependent relaxation to ACh in the absence or presence of NO synthase inhibitor L-NAME (). (d, e) Representative immunofluorescence staining images and statistical analysis for the protein level of eNOS (), DAPI (blue), and eNOS (green). (f, g) Western blot analysis of the protein level of eNOS with quantification (). (h) The level of NO was tested using the Griess reaction assay (NO reaction end products: nitrate plus nitrite) (). , vs. control group. #, ##, vs. DM group.