Functional Redox Proteomics Reveal That Salvia miltiorrhiza Aqueous Extract Alleviates Adriamycin-Induced Cardiomyopathy via Inhibiting ROS-Dependent Apoptosis
Table 1
List of identified oxidation of proteins in rat heart tissue.
Spot no.
Protein name
Accession number
Mw/pI
Score (coverage)1
Match fragment
Location
Function
1
ACON
Q9ER34
86.121/7.87
175 (37%)
23
Mitochondrion
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
2
GRP75
P48721
74.097/5.97
189 (49%)
24
Mitochondrion
May play a role in the control of cell proliferation and cellular aging.
3
PCCA
P14882
82.198/7.59
113 (32%)
16
Mitochondrion matrix
This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites.
4
ENOA
P04764
47.440/6.16
171 (54%)
18
Cytoplasm or cell membrane.
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses.
5
ALDH2
P11884
56.966/6.63
125 (36%)
14
Mitochondrion matrix
An aldehyde + H2O + NAD+ = a carboxylate + H+ + NADH
6
KCRM
P00564
43.246/6.58
78 (26%)
9
Cytoplasm.
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain, and spermatozoa.
7
ODPA
P26284
43.883/8.49
56 (20%)
8
Mitochondrion matrix
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
