Nrf2 and STAT3 Alleviates Ferroptosis-Mediated IIR-ALI by Regulating SLC7A11

Figure 1

Ferroptosis occurred in ALI due to IIR. (a) Endogenous levels of Fe of the lung tissue in the IIR-ALI model was measured with an iron assay. , , and between the groups. (b) Mice were divided into four groups: sham, IIR, IIR+Fe, and IIR+Fer-1. HE staining of the lung tissues (). (c, d) The lung pathological damage score following the administration of Fe (15 mg/kg) and Fer-1 (5 mg/kg), respectively ( per group). (e) GSH level in each group was measured by GSH assay ( per group). (f) MDA level in each group was measured by MDA assay ( per group). (g) Representative TEM images. The white arrow indicates the ultrastructure of the mitochondria in the sham group. The black arrows indicate the reduction or disappearance of mitochondrial cristae in the IIR group ( per group). (h) The level of lipid peroxidation-associated index, GPX4, in each group and the representative quantification of GPX4 protein. , , , ^#, ^##, and ^### between the groups. Compared with the sham group. ^#Compared with the IIR group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)