Nrf2 and STAT3 Alleviates Ferroptosis-Mediated IIR-ALI by Regulating SLC7A11

Figure 2

The lipid peroxidation index associated with ferroptosis was significantly altered in IIR-induced ALI. The mice were divided into four groups: sham, IIR, IIR+Fe, and IIR+Fer-1 after administrating Fe (15 mg/kg) and Fer-1 (5 mg/kg), respectively. (a) The level Nrf2 protein expression in each group was measured with a western blot and the representative quantification of Nrf2 protein ( per group). (b) The level of GPX4 in WT and Nrf2^-/- group and the representative quantification of GPX4 protein ( per group). (c) The GSH level in the WT and Nrf2^-/- group ( per group). (d) The MDA level in WT and Nrf2^-/- group ( per group). (e) Representative TEM images of WT and Nrf2^-/- mice. The white arrow indicates the ultrastructure of the mitochondria in the sham group, and the black arrows indicate the reduction or disappearance of mitochondrial cristae in the IIR group ( per group). , , , ^#, and ^## between the groups. Compared with the sham group. ^#Compared with the IIR group.

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