Nrf2 and STAT3 Alleviates Ferroptosis-Mediated IIR-ALI by Regulating SLC7A11

Figure 3

pSTAT3 and the downstream protein, SLC7A11, were significantly increased in IIR-ALI and regulated by Nrf2. (a) The level of STAT3 mRNA in the Nrf2^-/- mice compared with WT. , , , ^#, and ^## between the groups. Compared with the sham group; ^#Nrf2^-/-compared with that of the WT group ( per group). (b) WT and Nrf2^-/- mice were divided into four groups: sham, IIR, IIR+Fe, and IIR+Fer-1. The level of STAT3, pSTAT3, and SLC7A11protein expression was measured using a western blot. (c) The representative quantification of pSTAT3 protein, normalized to the corresponding level of total STAT3 ( per group). (d) The representative quantification of SLC7A11 protein, normalized to the corresponding level of β-actin ( per group). , , ^#, and ^## between the groups. Compared with the sham group; ^#Nrf2^-/-compared with that of the WT group.

(a)

(b)

(c)

(d)