Nrf2 and STAT3 Alleviates Ferroptosis-Mediated IIR-ALI by Regulating SLC7A11
STAT3 overexpression may alleviate the OGD/R-induced lung epithelial cell ferroptosis. Cells transfected with the STAT3 overexpressing lentivirus were subjected to OGD/R. (a) Relative level of STAT3, pSTAT3, and SLC7A11 protein expression after STAT3 overexpression. The pSTAT3 intensity was normalized to the corresponding level of STAT3. The STAT3 and SLC7A11 intensity were normalized to the corresponding level of β-actin, and the changes are shown in the histograms. ,, and between the groups. oeSTAT3 group compared with that of the oeNC group. (b) The cell proliferation activity was measured using a CCK8 assay after establishing OGD/R and treating with Fe or Fer-1. ,, and between the groups. oeSTAT3 group compared with that of the oeNC group. (c, d) The level of GSH and MDA in the STAT3 overexpressing cells after OGD/R; ,,,,, and between the groups. OGD/R group compared with the control group; #oeSTAT3 group compared with that of the oeNC group. (e) Relative level of GPX4 protein after overexpressing STAT3. ,,,#,##, and ### between the groups. OGD/R group compared with control group; #oeSTAT3 group compared with that of the oeNC group.
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