Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 5

Research Article

The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling

Figure 5

Assessment of DNA damage induced by 4-HPPP. (a) H1299 cells were administered the indicated concentrations of 4-HPPP for 48 h. Afterward, 4-HPPP-induced DNA damage was detected using a flow cytometry-based γH2AX detection assay. The green fluorescence of FITC (FL1) indicates the γH2AX-positive population (R1 region). The data showed that 4-HPPP increased the phosphorylation of γH2AX, a marker of DNA damage, in a dose-responsive manner. (b) Quantification analysis of (a). γH2AX was observed in H1299 cells following 4-HPPP treatment at concentrations from 0.5 to 10 μM by the Western blotting assay (c) and immunofluorescence assay (d). The data are presented as the . ; ; . Scale bar: 100 μm.

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