Glutamate Attenuates the Survival Property of IGFR through NR2B Containing N-Methyl-D-aspartate Receptors in Cortical Neurons

Figure 1

Glutamate decreases tyrosine phosphorylation of the IGF-1R and the prosurvival effect of IGF-1 in cultured cortical neurons. (a) Primary cultured cortical neurons were pretreated with 1 mM glutamate for 1 h and then exposed to 100 ng/ml IGF-1 for 8 min. Glutamate blocked the tyrosine phosphorylation of IGF-1R in a dose-dependent manner. Data represent assays from at least three independent experiments. (b) Cultured cortical neurons were pretreated with 1 mM glutamate, and then, cells were exposed to 100 ng/ml IGF-1 for 48 h and the cell viability was determined. Glutamate inhibited the prosurvival effect of IGF-1 in cultured cortical neurons. ; independent experiments.

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