Glutamate Attenuates the Survival Property of IGFR through NR2B Containing N-Methyl-D-aspartate Receptors in Cortical Neurons

Figure 3

Glutamate attenuates tyrosine phosphorylation and the survival/protective effect of IGF-1R through NMDARs. (a) Cultured cortical neurons were pretreated with the NMDA receptor antagonists MK801 or AP-5 (two selective NMDA receptor antagonists), DNQX (antagonist for the non-NMDA receptor, AMPA, and kainate receptors), CPCCOEt (selective, non-competitive mGluI antagonist), or LY341495 (mGluIII/II antagonist), and the effect of glutamate (1 mM) on IGF-1R phosphorylation was measured. Data represent assays from at least three independent experiments. (b) Cultured cortical neurons were pretreated with the NMDA receptor antagonists MK801 or AP-5, DNQX, CPCCOEt, or LY341495, and the effect of glutamate (1 mM) on the survival effect of IGF-1 was measured. Data represent assays from at least three independent experiments. .

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