Oxidative Medicine and Cellular Longevity / 2020 / Article / Tab 1 / Research Article
Integrated Metabolomic and Lipidomic Analysis Reveals the Neuroprotective Mechanisms of Bushen Tiansui Formula in an Aβ 1-42-Induced Rat Model of Alzheimer’s Disease Table 1 Differentially expressed endogenous metabolites between groups sham and AD and their change trends in all groups.
Metabolite Rt (min) HMDB ID PubChem ID AD vs. sham ADa BSTSFb VIP FC ESI+ Tangeritin 373.1267 5.3139 0030539 68077 2.374 0.001 0.591 ↓ ↑ Cer(d18:1/16:0) 538.5199 11.3614 0000790 5283564 1.953 0.004 0.750 ↓ ↑ Candicidin 566.289 7.6092 0015283 10079874 1.493 0.031 0.835 ↓ ↑ Cer(d18:1/24:1(15Z)) 648.6289 13.1778 0004953 5283568 1.468 0.035 0.871 ↓ — GlcCer(d14:1/20:0) 700.5725 11.005 — 70699223 1.257 0.043 0.883 ↓ ↑ Galbeta-Cer(d18:1/20:0) 778.6169 12.1307 — 44260150 1.564 0.012 0.902 ↓ ↑ Cer(d18:1/18:0) 566.5511 11.9439 0004950 5283565 2.03 0.004 0.929 ↓ — Sphinganine 302.3053 7.4398 0000269 91486 1.656 0.014 0.949 ↓ — LysoPE(16:1(9Z)/0:0) 452.2772 7.6754 0011504 52925129 1.644 0.021 0.976 ↓ ↑ PE(18:1(9Z)/16:0) 759.565 8.4109 0009055 9546802 2.422 0.001 0.977 ↓ — LysoPC(16:1(9Z)/0:0) 494.3241 7.5904 0010383 24779461 1.4 0.041 0.986 ↓ ↑ PI(20:4(5Z,8Z,11Z,14Z)/0:0) 621.303 7.8276 — 42607497 1.679 0.023 0.986 ↓ ↑ LysoPE(0:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 548.2745 7.6944 0011496 53480945 1.603 0.043 0.989 ↓ ↑ Oleamide 563.5505 8.9533 0002117 5283387 2.205 0.001 1.003 ↑ — Cer(d18:0/22:0) 665.6552 10.5332 0011765 5283575 1.564 0.01 1.004 ↑ ↓ Desmethylclomipramine 301.1428 13.9123 0060947 622606 1.551 0.026 1.005 ↑ ↓ Phytol 360.3263 13.3117 0002019 5280435 1.649 0.017 1.007 ↑ ↓ PE(15:0/14:1(9Z)) 680.4797 10.8995 0008888 52924158 1.879 0.014 1.017 ↑ ↓ Cotinine glucuronide 416.1429 0.768 0001013 3398121 1.78 0.025 1.024 ↑ ↓ 13Z-Docosenamide 338.3424 9.7988 — 5365371 1.091 0.05 1.027 ↑ ↓ PI(18:0/0:0) 601.3349 9.4901 — 42607495 1.735 0.011 1.027 ↑ — L-a-Lysophosphatidylserine 526.3142 9.1446 — 28040605 1.827 0.002 1.029 ↑ — S-Adenosylmethionine 399.1444 0.5786 0001185 34755 1.358 0.033 1.038 ↑ ↓ Rollinecin A 663.4538 11.419 0030438 177320 1.881 0.005 1.046 ↑ — Jubanine B 762.3905 6.2048 0030206 101316795 1.692 0.011 1.052 ↑ ↓ Sinapoylspermine 409.2812 6.1949 0033479 131751433 1.891 0.005 1.052 ↑ ↓ Latrepirdine 352.2404 12.5435 0240240 197033 1.828 0.002 1.059 ↑ ↓ Ecgonine methyl ester 232.1546 12.0323 0006406 104904 2.296 0.001 1.085 ↑ — Xestoaminol C 230.248 6.1084 — 14756407 1.128 0.044 1.087 ↑ ↓ Fasciculic acid B 678.4588 7.1307 0036438 196808 1.434 0.011 1.140 ↑ ↓ ESI- Citbismine A 639.1921 3.8775 0041086 131753020 1.875 0.007 0.840 ↓ ↑ Cer(d18:1/20:0) 638.5713 12.6161 0004951 5283566 2.051 0.004 0.916 ↓ ↑ Glucosylceramide (d18:1/18:0) 772.5938 11.5561 0004972 11958364 1.543 0.009 0.929 ↓ ↑ 1-O-Beta-D-glucopyranosyl-2,3-di-O-palmitoylglycerol 775.5571 11.6171 0031680 10462651 1.902 0.005 0.941 ↓ — PE(16:1(9Z)/P-16:0) 672.4954 11.2227 0008982 53479605 1.331 0.038 0.954 ↓ — PC(18:1(11Z)/18:2(9Z,12Z)) 771.5158 10.4438 0010620 53480619 1.676 0.027 0.962 ↓ ↑ PE(15:0/22:0) 806.5909 11.8787 0008907 52924172 1.69 0.025 0.974 ↓ — PS(20:1(11Z)/18:0) 838.5596 12.6161 0112545 52925649 2.134 0.006 0.977 ↓ — 4-Nitrophenol 138.02 3.6423 0001232 980 1.926 0.015 1.014 ↓ ↑ PC(18:1(11Z)/20:4(5Z,8Z,11Z,14Z)) 852.5756 10.6464 0008081 53478741 1.665 0.019 1.019 ↑ ↓ PE(18:3(9Z,12Z,15Z)/22:1(13Z)) 840.5778 10.9036 0009172 53479688 1.712 0.01 1.022 ↑ GDP 442.0154 0.883 0001201 135398619 1.617 0.006 1.042 ↑ — Luteolin 6-C-glucoside 8-C-arabinoside 655.1563 11.4852 0029258 131750830 1.708 0.046 1.067 ↑ ↓ Digoxigenin bisdigitoxoside 631.3473 10.9633 0060818 92999 1.397 0.045 1.155 ↑ ↓ Blasticidin S 459.1466 4.0897 0030452 170012 1.823 0.016 1.221 ↑ — Cefotaxime 490.0302 4.0897 0014636 5742673 1.656 0.043 1.715 ↑ — Deacetylnomilin 509.1563 3.6723 0035684 90472146 1.548 0.018 0.591 ↓ —
Abbreviations: AD: Alzheimer’s disease; Rt (min): retention time; VIP: variable importance; FC: fold change. Fold change was calculated as relative intensity obtained from group sham/group AD, and a value less than 1 indicates a decrease in the metabolites of group AD. The levels of potential biomarkers were labeled with “↓” (downregulated) and “↑” (upregulated) (
). a Change trend compared with the sham group.
b Change trend compared with the AD group.