Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 5

Research Article

Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells

Figure 5

Trehalose promotes LAMP2 expression independent of macroautophagy in human RPE cells. (a, b) Immunoblots showing an upregulation of LAMP2 expression by autophagy inhibitors chloroquine (CQ) and NH4Cl, and shRNA ATG5 knockdown. (c) RT-PCR evaluation of ATG5 knockdown. (d) Immunoblots showing increased LAMP2 in ATG5 knockdown cells without affecting macroautophagy. Data represent the of 3 independent experiments. Statistical analysis was performed by one-way ANOVA followed by multiple comparison tests. , vs. control.

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