Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells
Cytoprotection of trehalose against oxidative damage by hydroquinone (HQ) in ARPE-19 cells. (a) Trypan blue assay reveals that trehalose is nontoxic over a wide dose range. (b) Trehalose decreased basal level of reactive oxygen species (ROS) assessed using CM-H2DCFDA assay (c, d) Trehalose pretreatment improves the viability of cells and lowered the protein carbonyl levels in cells exposed to HQ. Data represent the of 3 independent experiments. Statistical analysis was performed by one-way ANOVA test, followed by multiple comparison post hoc test; ,.
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