Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 7

Research Article

Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells

Figure 7

Cytoprotection of trehalose against oxidative damage by hydroquinone (HQ) in ARPE-19 cells. (a) Trypan blue assay reveals that trehalose is nontoxic over a wide dose range. (b) Trehalose decreased basal level of reactive oxygen species (ROS) assessed using CM-H2DCFDA assay (c, d) Trehalose pretreatment improves the viability of cells and lowered the protein carbonyl levels in cells exposed to HQ. Data represent the of 3 independent experiments. Statistical analysis was performed by one-way ANOVA test, followed by multiple comparison post hoc test; , .
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