Tanshinone IIA Protects against Acute Pancreatitis in Mice by Inhibiting Oxidative Stress via the Nrf2/ROS Pathway

Figure 2

TSA relieved the oxidative stress injury and mitochondrial damage in pancreatic tissue in caerulein-induced AP. (a) Representative DHE immunofluorescence image of pancreatic tissues in magnification 100 μm. (b) DHE fluorescence. Percentage of V/S mean. (c) Levels of oxidative stress products (MDA and GSH) of pancreatic tissues. (d, e) Protein levels of Nrf2 and total HO-1 in pancreatic tissues were analyzed by western blotting. (f) The organelles of pancreatic acinar cells via transmission electron microscopy. Red and yellow arrowheads indicate mitochondria and endoplasmic reticulum, respectively. , , and versus the V/Cae group. each group. V represents vehicle; S represents saline; L, M, and H represent low-dose (5 mg/kg), medium-dose (25 mg/kg), and high-dose (50 mg/kg) TSA.

(a)

(b)

(c)

(d)

(e)

(f)