Cholesterol Induces Nrf-2- and HIF-1α-Dependent Hepatocyte Proliferation and Liver Regeneration to Ameliorate Bile Acid Toxicity in Mouse Models of NASH and Fibrosis

Figure 5

Inflammatory response in liver tissue. (a) IHC staining and analysis of inflammatory genes from liver tissue of wild-type mice supplemented with atherogenic diet constituents. IHC staining performed with antimacrophage marker F4/80. (1) Control group, (2) CHOL group, (3) CA group, and (4) CHOL+CA group: significant macrophage infiltration. . Gene expression: SAA1 and SAA2 relative mRNA levels and iNOS relative protein levels. All values are expressed as (). (b) Inflammatory gene expression and IHC of liver tissue of Mdr2-/- mice performed with staining of macrophage marker F4/80. (1) Mdr2-/- Control group: brown areas stained with DAB represent slight macrophage infiltration. (2) Mdr2-/- CHOL group: major brown staining with DAB represents multiple, peripheral macrophage clusters. . Gene expression: SAA1, SAA2, and iNOS mRNA levels. All values are expressed as (). Means with different letters are statistically different, .

(a)

(b)