Cellular Mechanisms Triggered by the Cotreatment of Resveratrol and Doxorubicin in Breast Cancer: A Translational In Vitro–In Silico Model

Figure 4

Strategy to predict a translational chemical-protein (CP) and protein-protein interacting (PPI) networks between MCF7 cell line and breast cancer. (a) MCF7 PPI network: based on the constitutive gene expression of MCF7, a PPI network was predicted using the STRING software. (b) MCF7 CP-PPI network: targets of Rsv and Doxo were predicted using the STITCH platform. (c) Breast cancer PPI network: the 1000 most significant genes associated with the term “malignant breast cancer” from DISEASES resource were used to perform a PPI network based on interacting data available from stringApp. (d) Breast cancer CP-PPI network: to predict targets of Rsv and Doxo, interactions were prospected using the STITCH platform. (e) Intersection CP-PPI network: an “intersection” operation, available in the NetworkAnalyzer, was performed to obtain common nodes and edges between the MCF7 CP-PPI network and the breast cancer CP-PPI network.