Dexmedetomidine Alleviates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting p75NTR-Mediated Oxidative Stress and Apoptosis

Figure 3

Dexmedetomidine suppressed apoptosis in AKI induced by LPS. (a, b) TUNEL staining in kidney sections of mice (), white arrows to show TUNEL-positive nuclei and quantitation of TUNEL-positive cells. (c, d) Cleaved caspase-3 staining in kidney sections of mice (), and quantitation of Cleaved caspase-3-positive cells. (e, f) Western blot analysis and quantitative data for Cleaved caspase-3, p75NTR, proNGF, and NGF in kidney tissues of mice; GAPDH was used as a loading control. (g) p75NTR, NGF, TrkA, and Sortilin gene expression in the kidney tissues of mice ( and versus control; ^# and ^## versus LPS. Data were presented as , ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)