Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 5

Research Article

O-GlcNAcylation Is Essential for Autophagy in Cardiomyocytes

Figure 5

ULK1 is O-GlcNAcylated in cardiomyocytes. (a) Left panel, western blot analysis of Ad-LacZ- and Ad-Cre-infected Ogtf/f and/or Ogtf/y neonatal cardiomyocytes, using antibodies against O-GlcNAc and ULK1. GAPDH was detected as a loading control. Right panel, quantification of western blot results in the left panel, for each group. Significantly different; M.W.: molecular weight. (b) Immunoprecipitation of Ad-LacZ- and Ad-Cre-infected Ogtf/f and/or Ogtf/y neonatal cardiomyocytes using antibody against O-GLcNAc (RL2), followed by western blot using antibodies against O-GlcNAc (RL2) and ULK1. Ab.: antibody; M.W.: molecular weight. (c) Immunoprecipitation of Ad-LacZ- and Ad-Cre-infected Ogtf/f and/or Ogtf/y neonatal cardiomyocytes using antibody against ULK1, followed by western blot using antibodies against O-GlcNAc (RL2) and ULK1. Ab.: antibody; M.W.: molecular weight. N.S.: nonspecific band.
(a)
(b)
(c)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.