O-GlcNAcylation Is Essential for Autophagy in Cardiomyocytes

Figure 5

ULK1 is O-GlcNAcylated in cardiomyocytes. (a) Left panel, western blot analysis of Ad-LacZ- and Ad-Cre-infected Ogt^f/f and/or Ogt^f/y neonatal cardiomyocytes, using antibodies against O-GlcNAc and ULK1. GAPDH was detected as a loading control. Right panel, quantification of western blot results in the left panel, for each group. Significantly different; M.W.: molecular weight. (b) Immunoprecipitation of Ad-LacZ- and Ad-Cre-infected Ogt^f/f and/or Ogt^f/y neonatal cardiomyocytes using antibody against O-GLcNAc (RL2), followed by western blot using antibodies against O-GlcNAc (RL2) and ULK1. Ab.: antibody; M.W.: molecular weight. (c) Immunoprecipitation of Ad-LacZ- and Ad-Cre-infected Ogt^f/f and/or Ogt^f/y neonatal cardiomyocytes using antibody against ULK1, followed by western blot using antibodies against O-GlcNAc (RL2) and ULK1. Ab.: antibody; M.W.: molecular weight. N.S.: nonspecific band.

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