Hypoxia-Induced Glioma-Derived Exosomal miRNA-199a-3p Promotes Ischemic Injury of Peritumoral Neurons by Inhibiting the mTOR Pathway

Figure 6

NHIGDE could aggravate OGD injury in primary neurons by overexpressing miRNA-199a-3p. (a, b) When primary neurons were treated with NHIGDE+miRNA-199a-3p mimics, the mRNA and protein levels of the mTOR pathway in primary neurons decreased compared with those in neurons treated with NHIGDE+vehicle, similar to neurons treated with HIGDE+vehicle after applied OGD/reperfusion. vs. NHIGDE+vehicle. In contrast, (c) before and (d) after OGD/reperfusion injury, the mRNA and protein levels of the mTOR pathway were higher in primary neurons cultured with HIGDE from C6 cells treated with miRNA-199a-3p shRNA than in neurons treated with HIGDE+scramble shRNA, similar to neurons treated with NHIGDE. vs. NHIGDE; ^# vs. scramble shRNA+HIGDE. /group in at least 3 independent experiments.

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