Hypoxia-Induced Glioma-Derived Exosomal miRNA-199a-3p Promotes Ischemic Injury of Peritumoral Neurons by Inhibiting the mTOR Pathway

Figure 7

The upregulation of miRNA-199a-3p in HIGDE was mediated by hypoxia-induced HIF-1α activation. (a) Bioinformatics analysis identified HIF-1α reacting element (HRE) sequences in the upstream area of miRNA-199a1. (b) Western blotting further confirmed the upregulated expression of HIF-1α in C6 cells cultured in hypoxia-treated environments. (c) The expression of miRNA-199a-3p RNA in NHIGDE+Lenti-HIF-1α was remarkably higher than that in NHIGDE without HIF-1α overexpression (Lenti GFP). vs. Lenti GFP. (d) When the expression of HIF-1α in C6 cells was knocked down (HIF-1α shRNA), the expression of miRNA-199a-3p in HIGDE decreased significantly in comparison to that in HIGDE without HIF-1α suppression. vs. HIGDE+scramble shRNA. /group in at least 3 independent experiments.

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