Monoclonal Antibody to CD14, TLR4, or CD11b: Impact of Epitope and Isotype Specificity on ROS Generation by Human Granulocytes and Monocytes

Table 3

The capability of antibodies against CD11b/CD18 affects the LPS-induced effects (the references are indicated inside the square brackets).

Clone (isotype)	Epitope	Influence on LPS-induced effects		References
		Does	Does not
OKM-1 (mIgG2b)	CD11b AA614–682 (lectin site)	(1) Increase TNF-α production from monocytes stimulated by LPS S. minnesota (1 ng/ml, without serum)	(1) Inhibit binding of LPS E. coli O111:B4 (10 ng/ml, 10% FCS) to human PBMC; (2) Inhibit PMN priming by LPS E. coli O55:B5 (100 ng/ml, 1% serum) for fMLP-triggered О2^⋅−/ROS production; (3) Inhibit protein (41 and 42 kDa) tyrosine phosphorylation in macrophages stimulated by Re-LPS S. minnesota R595 (1 ng/ml, human serum); (4) Influence on О2^⋅−/ROS production induced by Re-LPS E. coli (1 ng/ml, 5% serum) in human monocytes; (5) Influence on TNF-α production from human PBMC stimulated by LPS E. coli O111:B4 (100 ng/ml, 10% serum)	[63, 91, 99, 100, 104, 105, 157, 158]
904 (mIgG2b)	CD11b AA74–316 (αA/I-domain)	(1) Suppress macrophage interaction with bovine erythrocytes opsonized by Re-LPS S. minnesota R595		[159, 160]
ICRF 44 (mIgG1)	CD11b		(1) Affect PMN priming by S- or Re-LPS E. coli (100 ng/ml, 2% serum) for fMLP-triggered О2^⋅−/ROS production	[13]
IB4 (mIgG2a)	CD11b/CD18	(1) Increase TNF-α production from monocytes stimulated by LPS S. minnesota (1 ng/ml, without serum)	(1) Inhibit binding of LPS E. coli O111:B4 (100 ng/ml, 10% serum) to human PBMC and TNF-α production; (2) Influence on activation of p38 MAPK during PMN priming by LPS E. coli O55:B5 (5 ng/ml) for fMLP-triggered О2^⋅−/ROS production	[77, 99, 157]