Yolkin Isolated from Hen Egg Yolk as a Natural Immunoregulator, Activating Innate Immune Response in BMDM Macrophages

Figure 3

Effect of yolkin on nitric oxide production in BMDM cells. BMDM cells () were treated with yolkin (10, 100, and 150 μg/ml) or LPS (1 μg/ml) in the presence or absence of an S-MIU inhibitor (10 μM) for 24 h. Thereafter, supernatants were collected and the level of NO was detected by the Griess reaction. The difference in the level of NO in control and yolkin-treated cells was significant (). Results represent three independent experiments, and data are presented here as .