Yolkin Isolated from Hen Egg Yolk as a Natural Immunoregulator, Activating Innate Immune Response in BMDM Macrophages

Figure 5

Upregulation of type I interferon expression (a) and production (b) after yolkin treatment of BMDM cells. (a) BMDM cells () were cultured alone or stimulated with yolkin (100 μg/ml) or with LPS (1 μg/ml). After 4 h of stimulation, total RNA was isolated from the cells and expression of type I IFN mRNA was determined by real-time PCR. (b) BMDM cells () were either treated with yolkin (10, 100, and 150 μg/ml) or LPS (1 μg/ml) or left untreated for 24 h. Supernatants were collected, and the level of type I interferons was determined by bioassay. Results are presented as (). : statistically significant difference in value versus control.

(a)

(b)