Yolkin Isolated from Hen Egg Yolk as a Natural Immunoregulator, Activating Innate Immune Response in BMDM Macrophages

Figure 9

Evaluation of the antiviral activity of yolkin in the BMDM mouse macrophage model. A 24 hour preincubation of BMDM cells with yolkin at concentrations of 100 μg/ml and 150 μg/ml stimulates cells to produce type I IFNs which in turn inhibits VSV replication. Option I—medium above BMDM cells stimulated with yolkin at concentrations of 100 μg/ml and 150 μg/ml was added to additional BMDM cells simultaneously with VSV with a dose of 100 TCID₅₀/ml. Option II—medium above BMDM cells stimulated with yolkin at concentrations of 100 μg/ml and 150 μg/ml was added to additional BMDM cells after VSV infection with a dose of 100 TCID₅₀/ml.