The Role of DNMT and HDACs in the Fetal Programming of Hypertension by Glucocorticoids

Table 1

Primer specification and qPCR parameters.

Gene target	Primer sequences	Amplicon size (bp)	Amplification conditions	cDNA input (ng)	Primer input (nM)
PAH; NM_012619.2	F:GCTGCTAAGCTAGACACCTCA; R:CTTGTTTCCTGCCCAAAGTCT	105	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 60°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 39 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	600
TH; L22651	F:GCGACAGAGTCTCATCGAGGAT R:AGAGCAGGTTGAGAACAGCATT	150	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 58°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 29 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	600
DBH; NM_013158	F:TTCCCCATGTTCAACGGACC; R:GCTGTGTAGTGTAGACGGATGC	240	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 58°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 29 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	600
PNMT; X75333	F:CATCGAGGACAAGGGAGAGTC; R:GCAGCGTCGTGATATGATAC	219	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 60°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 39 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	300
SP1; D12768.1	F:CAGACTAGCAGCAGCAATACCA R:TGAAGGCCAAGTTGAGCTCCAT	224	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 58°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 29 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	600
EGR1; AY551092.1	F:TTTCCACAACAACAGGGAGAC; R:CTCAACAGGGCAAGCATACG	261	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 58°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 29 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	600
GR; NM_012576.2	F:TGCTGGAGGTGATTGAACCC; R:TCACTTGACGCCCACCTAAC	111	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 58°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 29 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	600
Beta actin; NM_031144	F:TCTGTGTGGATTGGTGGCTCT; R:GACTCATCGTACTCCTGCTTG	83	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 58°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 29 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	600
RPL29; NM_017150	F:TGAGAGGTAGGGTCCCGTTT; R:TCAGTTCTGGGACCTGACCA	144	1. 95°C, 2 min; 2. 95°C, 1 min; 3. 58°C, 1 min; 4. 72°C, 1 min; 5. Plate read; 6. Go to line 2, 29 more times; 7. Melting curve from 55-95°C, read every 1°C, hold 10 sec	7.5	600