Network Pharmacology Analysis and Molecular Characterization of the Herbal Medicine Formulation Qi-Fu-Yin for the Inhibition of the Neuroinflammatory Biomarker iNOS in Microglial BV-2 Cells: Implication for the Treatment of Alzheimer’s Disease

Figure 5

Chemical characterization of fraction 9 derived from 90% RS. (a) Chromatographic profiles of the parent 90% RS solution and the derived fraction 9. (b) Verification of ginsenoside Rg3 in the 90% RS-derived fraction 9. Fraction 9 and ginsenoside Rg3, alone or in combination, were analyzed by HPLC-MS/MS on a C18 column under the same conditions. (c) Bioassays of ginsenoside Rg3 for suppressing iNOS induction. BV-2 cells were treated with the indicated concentration of 90% QFY, 90% RS, and commercial ginsenoside Rg3 for 24 h. The expression of iNOS was detected by Western blot, and the blots were quantified as previously described. (LPS vs. untreated control); , (treatment+LPS vs. LPS).

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