Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 1

Research Article

Drp-1 as Potential Therapeutic Target for Lipopolysaccharide-Induced Vascular Hyperpermeability

Figure 1

Depletion of Drp-1 exacerbates LPS-induced cell apoptosis. PMVECs were transfected with Drp-1 siRNA (a scrambled siRNA was used as the control) and exposed to LPS (500 ng/ml) for 24 h. (a) Western blotting evaluation of Drp-1 levels in cytosolic and mitochondrial cell fractions. (b) Mitochondrial Drp-1 level quantification; (c) cytoplasmic Drp-1 level quantification; (d) cellular apoptosis was assessed using TUNEL staining. Scale bars, 50 μm. (e) The number of TUNEL-positive cells per field. The represent the data ( in each group). An asterisk () indicates vs. the various groups. Drp-1: Dynamin-related protein-1; LPS: lipopolysaccharide; 3MA: 3-Methyladenine; TUNEL: terminal deoxynucleotidyl transferase nick-end labeling; COX IV: cytochrome C oxidase subunit 4.

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