Interaction of TPPP3 with VDAC1 Promotes Endothelial Injury through Activation of Reactive Oxygen Species

Figure 2

Influence of TPPP3 in PA-induced endothelial injuries in HUVECs. HUVECs were transduced with SCR (scrambled shRNA) or TPPP3 shRNAs and then treated with 200 μM PA for 24 h before the following analyses. (a) TPPP3, BCL-2, Caspase-3, Caspase-9, Cytochrome C, and Bax protein level in HUVECs after TPPP3 silencing measured by Western blot. (b) Flow cytometry analysis, (c) representative images of immunostaining, and (d) Matrigel tube formation assay of HUVECs as indicated treatment. Flow cytometry analysis of cell apoptosis (e) and representative images of ROS staining (f) showed cell apoptosis and production of ROS in TPPP3 overexpressed HUVECs treated with 50 μM PA. (g) Tube formation assay showed overexpression of TPPP3 aggravated PA-induced impairment of endothelial tube formation. Student’s -test or one-way ANOVA was performed to assess the difference. vs. control, ^# vs. SCR.

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