Research Article
Interaction of TPPP3 with VDAC1 Promotes Endothelial Injury through Activation of Reactive Oxygen Species
Figure 5
Role of ROS in TPPP3-induced cell dysfunction in HUVECs. HUVECs overexpressing TPPP3 were treated with 5 μM NAC or control or without any treatment for 2 h and then incubated with 50 μM PA for 24 hours. (a) The cell lysates were analyzed by Western blot using antibodies against TPPP3, VDAC1, BCL-2, Bax, Cyto C, Caspase-3, and Caspase-9. Apoptosis analysis (b), production of ROS (c), and capillary-like tubes (d) were detected by flow cytometry, DCFDA assay, and tube formation assay, respectively. One-way ANOVA was performed to assess the difference. .
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