Quadrella incana (Capparaceae) Leaf Extract Enhances Proliferation and Maintenance of Neural Stem/Progenitor Cells through Upregulating Glycolytic Flux and Redox Potential

Figure 1

Quadrella incana leaf extract enhanced the proliferation and inhibited spontaneous differentiation of NSPCs in proliferating condition. (a) Western blot analysis of Bmi1, βIII tubulin, and phospho-Histone 3 (Ser 10) in NSPCs kept under proliferating media upon treatment for 3 days with DMSO, Quadrella incana leaf extract, or six different plant crude extracts. GAPDH was used as a loading control. GAPDH was used as a loading control. (b) Sphere-forming capacity of NSPCs treated with vehicle (DMSO) or 100 μg/ml Quadrella incana leaf extract for 3 days. . (c) Average diameters of suspended spheres treated with DMSO or 100 μg/ml Quadrella incana leaf extract for 3 days. The unpaired two-tailed Student’s test was used to determine the statistical significance. . (d) Morphology of NSPCs kept under proliferating media after treatment of DMSO or 100 μg/ml Quadrella incana leaf extract for 3 days. Arrows indicate cells attached to the bottom of the plate.

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