A Dihydroflavonoid Naringin Extends the Lifespan of C. elegans and Delays the Progression of Aging-Related Diseases in PD/AD Models via DAF-16

Figure 4

The effect of naringin on the lifespan extension in C. elegans depends on FOXO homologous daf-16. (a) Survival curves of daf-16 mutants in the control or treated with 50 μM naringin; naringin could not further extend the mean lifespan of C. elegans. (b) 50 μM naringin could not lead to DAF-16 nuclear localization. DAF-16::GFP expressing worms were placed on the plates with 50 μM naringin and control plates at 20°C for 48 h. (c) The mRNA level of genes downstream of daf-16 in worms (N2) treated with or without 50 μM naringin. The columns showed the mean value of two independent experiments with error bars representing SEM. represents , calculated using two-tailed -test. Statistical details and repeats of these experiments are summarized in Table S9 (Supplementary information). (d) Representative images of the SOD-3::GFP report transgenic CF1553 with naringin or not; naringin enhanced the fluorescence intensity of SOD-3. The aggregation of SOD-3 in N2 treated with or without naringin was captured with a Leica epifluorescence microscope and analyzed by using the image processing software ImageJ. Statistical details and repeats of these experiments are summarized in Table S10 (Supplementary information). (e) Representative images of the degree of ROS with wild-type (N2) animals raised at 20°C on NGM plates via naringin, NAC (5 mM), and H₂O₂ (2 mM) treated; naringin impaired the fluorescence intensity of ROS. The aggregation of ROS in N2 treated with or without naringin was captured with a Leica epifluorescence microscope and analyzed by using the image processing software ImageJ. Statistical details and repeats of these experiments are summarized in Table S10 (Supplementary information).

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