EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic db/db Mice and HK-2 Cells

<div>The protein expression of Klotho in HK-2 cells detected by western blotting. (a) Effect of different doses of EGCG on Klotho expression in HG-induced HK-2 cells. (b) The expression of Klotho/<i>β</i>-actin was quantitatively analyzed in each group. NG: normal glucose (5.5 mM); HG: high glucose (60 mM); EGCG0.1, EGCG1, EGCG20, and EGCG50: HK-2 cells treated with EGCG doses of 0.1, 1, 20, and 50 <i>μ</i>M in high-glucose conditions, respectively. 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic db/db Mice and HK-2 Cells 