Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 2

Research Article

AdipoRon Protects against Tubular Injury in Diabetic Nephropathy by Inhibiting Endoplasmic Reticulum Stress

Figure 2

Effects of AdipoRon on inflammation, apoptosis, and fibrosis in the kidneys of db/db mice. (a) Representative immunofluorescence images of collagen I (A–C) and FN (D–F) expression in the renal tissues of db/m, db/db, and db/db mice treated with AdipoRon. Oxidative stress and apoptosis in renal tissues were detected using DHE reagents (G–I) and TUNEL staining (J–L), respectively. mice per group. . (b) Semiquantitative analysis of the protein levels of collagen I and FN detected by IF. (c) Semiquantitative assessment of oxidative stress and apoptosis in each group. (d) Western blot analysis of cleaved caspase 3, Bcl-2, Bax, FN, and collagen I. β-Actin was used as a loading control. mice per group. (e) Relative band density of cleaved caspase 3, Bcl-2/Bax, FN, and collagen I expression. (f–h) mRNA levels of MCP-1, IL-6, and TNFα in the renal tissues of each group determined by qRT-PCR. The data are shown as the . versus db/m control; # versus db/db mice. FN: fibronectin; DHE: dihydroethidium; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; MCP-1: monocyte chemotactic protein-1; IL-6: interleukin-6; TNFα: tumor necrosis factor α; qRT-PCR: quantitative reverse-transcriptase PCR.

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