AdipoRon Protects against Tubular Injury in Diabetic Nephropathy by Inhibiting Endoplasmic Reticulum Stress

Figure 5

Effect of AdipoR1 siRNA, AICAR, and compound C on the levels of p-AMPK and ER stress in HK-2 cells treated with AdipoRon under HG conditions. (a) Immunoblot assays of the protein expression of p-AMPK, T-AMPK, GRP78, p-PERK, PERK, and CHOP in HK-2 cells treated with AdipoRon, AdipoR1 siRNA, AICAR, or compound C under HG ambience. per group. (b) Relative band density. (c) Representative western blot bands of Bcl-2, Bax, cleaved caspase 3, collagen I, and FN in HK-2 cells treated with AdipoRon, AdipoR1 siRNA, AICAR, or compound C under HG conditions. per group. (d) Relative band density. The data are shown as the . compared to the LG group; ^# compared to the HG group. ^$ compared to the HG+AdipoRon group. ^@ compared to the HG+AdipoRon+AdipoR1 siRNA group. ^& compared to the HG+AdipoRon group. AICAR: 5-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]imidazole-4-carboxamide.

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